Spore photoproduct lyase

Spore photoproduct lyase (EC 4.1.99.14, SAM, SP lyase, SPL, SplB, SplG) is a radical SAM enzyme that repairs lesions that arise upon UV-radiation of bacterial DNA. These lesions include cross linking at bipyrimidine sites between strands of DNA; this cross linking alters DNA structure and inhibits DNA replication. [1]

The repair mechanism utilizing spore photoproduct lyase is one of the reasons for the resilience of certain bacterial spores. Through a series of radical reactions the photodimer, 3 5-thyminyl-5,6-dihydrothymine, is disconnected to give back two functional thymine rings.[2][3]

Spore photoproduct lyase (reaction diagram).png

Spore photoproduct lyase is part of one of two main pathways which are used to repair cross linked 5-thyminyl-5,6-dihydrothymine caused by UV radiation: the spore-specific DNA repair system (which utilizes spore photoproduct lyase), and the general nucleotide excision repair pathway (NER). [1]

Spore photoproduct lyase
Identifiers
EC number4.1.99.14
CAS number37290-70-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum

References[]

  1. ^ a b Yang L, Li L (February 2015). "Spore photoproduct lyase: the known, the controversial, and the unknown". The Journal of Biological Chemistry. 290 (7): 4003–9. doi:10.1074/jbc.R114.573675. PMC 4326811. PMID 25477522.
  2. ^ Wang SC, Frey PA (March 2007). "S-adenosylmethionine as an oxidant: the radical SAM superfamily". Trends in Biochemical Sciences. 32 (3): 101–10. doi:10.1016/j.tibs.2007.01.002. PMID 17291766.Note that the SPL drawings are incorrect in this paper and the erratum
  3. ^ Buis JM, Cheek J, Kalliri E, Broderick JB (September 2006). "Characterization of an active spore photoproduct lyase, a DNA repair enzyme in the radical S-adenosylmethionine superfamily". The Journal of Biological Chemistry. 281 (36): 25994–6003. doi:10.1074/jbc.M603931200. PMID 16829680.

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