Chiral column chromatography

Chiral column chromatography is a variant of column chromatography that is employed for the separation of enantiomers, e. g. in racemates. The stationary phase contains a single enantiomer of a chiral compound.[1]

The chiral stationary phase can be prepared by attaching a chiral compound to the surface of an achiral support such as silica gel. Common chiral stationary phases are based on oligosaccharides such as cellulose or cyclodextrin (in particular with β-cyclodextrin, a seven sugar ring molecule).

The principle can be also applied to the fabrication of monolithic HPLC columns[2] or gas chromatography columns.[3]


  1. ^ Seebach, Dieter; Sting, Andrea R.; Hoffmann, Matthias (1996). "Self-Regeneration of Stereocenters(SRS)—Applications, Limitations, and Abandonment of a Synthetic Principle". Angewandte Chemie International Edition in English. 35 (2324): 2708–2748. doi:10.1002/anie.199627081.
  2. ^ Yingjie Li; Chunhui Song; Lingyi Zhang; Weibing Zhang; Honggang Fu (January 2010). "Fabrication and evaluation of chiral monolithic column modified by β-cyclodextrin derivatives". Talanta. 80 (3): 1378–1384. doi:10.1016/j.talanta.2009.09.039. PMID 20006102.
  3. ^ Yi-Ming Liu; Patricia Gordon; Shelby Green; Jonathan V. Sweedler (September 2000). "Determination of salsolinol enantiomers by gas chromatography-mass spectrometry with cyclodextrin chiral columns". Analytica Chimica Acta. 420 (1): 81–88. doi:10.1016/S0003-2670(00)00986-7.