(S)-N-methylcoclaurine,NADPH:oxygen oxidoreductase (3'-hydroxylating)

N-methylcoclaurine 3'-monooxygenase
EC no.
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum
Gene OntologyAmiGO / QuickGO

In enzymology, a N-methylcoclaurine 3'-monooxygenase (EC is an enzyme that catalyzes the chemical reaction

(S)-N-methylcoclaurine + NADPH + H+ + O2 (S)-3'-hydroxy-N-methylcoclaurine + NADP+ + H2O

The 4 substrates of this enzyme are (S)-N-methylcoclaurine, NADPH, H+, and O2, whereas its 3 products are (S)-3'-hydroxy-N-methylcoclaurine, NADP+, and H2O.

This enzyme belongs to the family of oxidoreductases, specifically those acting on paired donors, with O2 as oxidant and incorporation or reduction of oxygen. The oxygen incorporated need not be derived from O2 with NADH or NADPH as one donor, and incorporation of one atom of oxygen into the other donor. The systematic name of this enzyme class is (S)-N-methylcoclaurine,NADPH:oxygen oxidoreductase (3'-hydroxylating). This enzyme is also called N-methylcoclaurine 3'-hydroxylase and CYP80B1.[1]


  1. ^ Pauli HH, Kutchan TM (1998). "Molecular cloning and functional heterologous expression of two alleles encoding (S)-N-methylcoclaurine 3'-hydroxylase (CYP80B1), a new methyl jasmonate-inducible cytochrome P-450-dependent mono-oxygenase of benzylisoquinoline alkaloid biosynthesis". Plant J. 13 (6): 793–801. doi:10.1046/j.1365-313X.1998.00085.x. PMID 9681018.