(R,S)-2-haloacid dehalogenase (configuration-inverting)

2-haloacid dehalogenase (configuration-inverting)
EC no.
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum

2-haloacid dehalogenase (configuration-inverting) (EC, 2-haloalkanoic acid dehalogenase, 2-haloalkanoid acid halidohydrolase, DL-2-haloacid dehalogenase, DL-2-haloacid dehalogenase (inversion of configuration), DL-2-haloacid halidohydrolase (inversion of configuration), DL-DEXi, (R,S)-2-haloacid dehalogenase (configuration-inverting)) is an enzyme with systematic name (S)-2-haloacid dehalogenase (configuration-inverting).[1][2][3][4][5][6][7][8][9] This enzyme catalyses the following chemical reaction

(1) (S)-2-haloacid + H2O (R)-2-hydroxyacid + halide
(2) (R)-2-haloacid + H2O (S)-2-hydroxyacid + halide

This enzyme dehalogenates both (S)- and (R)-2-haloalkanoic acids.


  1. ^ Motosugi K, Esaki N, Soda K (May 1982). "Bacterial assimilation of D- and L-2-chloropropionates and occurrence of a new dehalogenase". Archives of Microbiology. 131 (3): 179–83. doi:10.1007/BF00405875. PMID 7103659.
  2. ^ Motosugi K, Esaki N, Soda K (May 1982). "Purification and properties of a new enzyme, DL-2-haloacid dehalogenase, from Pseudomonas sp". Journal of Bacteriology. 150 (2): 522–7. PMC 216397. PMID 7068529.
  3. ^ Motosugi K, Esaki N, Soda K (July 1984). "Enzymatic preparation of D- and L-lactic acid from racemic 2-chloropropionic acid". Biotechnology and Bioengineering. 26 (7): 805–6. doi:10.1002/bit.260260729. PMID 18553452.
  4. ^ Kurihara T, Esaki N, Soda K (2000). "Bacterial 2-haloacid dehalogenases: structures and reaction mechanisms". J. Mol. Catal., B Enzym. 10 (1–3): 57–65. doi:10.1016/S1381-1177(00)00108-9.
  5. ^ Liu JQ, Kurihara T, Hasan AK, Nardi-Dei V, Koshikawa H, Esaki N, Soda K (July 1994). "Purification and characterization of thermostable and nonthermostable 2-haloacid dehalogenases with different stereospecificities from Pseudomonas sp. strain YL". Applied and Environmental Microbiology. 60 (7): 2389–93. PMC 201661. PMID 8074519.
  6. ^ Cairns SS, Cornish A, Cooper RA (February 1996). "Cloning, sequencing and expression in Escherichia coli of two Rhizobium sp. genes encoding haloalkanoate dehalogenases of opposite stereospecificity". European Journal of Biochemistry. 235 (3): 744–9. doi:10.1111/j.1432-1033.1996.t01-1-00744.x. PMID 8654424.
  7. ^ Leigh JA, Skinner AJ, Cooper RA (1988). "Partial purification, stereospecificity and stoichiometry of three dehalogenases from a Rhizobium species". FEMS Microbiol. Lett. 49 (3): 353–356. doi:10.1111/j.1574-6968.1988.tb02756.x.
  8. ^ Weightman AJ, Weightman AL, Slater JH (August 1982). "Stereospecificity of 2-monochloropropionate dehalogenation by the two dehalogenases of Pseudomonas putida PP3: evidence for two different dehalogenation mechanisms". Journal of General Microbiology. 128 (8): 1755–62. doi:10.1099/00221287-128-8-1755. PMID 7142958.
  9. ^ Soda K, Kurihara T, Liu, JQ, Nardi-Dei V, Park C, Miyagi M, Tsunasawa S, Esaki N (1996). "Bacterial 2-haloacid dehalogenases: Structures and catalytic properties". Pure Appl. Chem. 68 (11): 2097–2103. doi:10.1351/pac199668112097.

External links[]