Thus, the two substrates of this enzyme are (7R)-7-(4-carboxybutanamido)cephalosporanate and H2O, whereas its two products are (7R)-7-aminocephalosporanate and glutarate.
This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides. The systematic name of this enzyme class is (7R)-7-(4-carboxybutanamido)cephalosporanate amidohydrolase. Other names in common use include 7beta-(4-carboxybutanamido)cephalosporanic acid acylase, cephalosporin C acylase, glutaryl-7-ACA acylase, CA, GCA, GA, cephalosporin acylase, glutaryl-7-aminocephalosporanic acid acylase, and GL-7-ACA acylase. This enzyme participates in penicillin and cephalosporin biosynthesis.
Shimomura K; Saito, Y; Fujimura, T; Sasaki, H; Noguchi, Y; Yamada, H; Niwa, M; Shimomura, K (1995). "High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176". Eur. J. Biochem.230 (2): 773–778. doi:10.1111/j.1432-1033.1995.0773h.x. PMID7607251.
Kim JK, Yang IS, Rhee S, Dauter Z, Lee YS, Park SS, Kim KH (2003). "Crystal structures of glutaryl 7-aminocephalosporanic acid acylase: insight into autoproteolytic activation". Biochemistry. 42 (14): 4084–4093. doi:10.1021/bi027181x. PMID12680762.