Fructan beta-(2,6)-fructosidase (EC 3.2.1.154 , beta-(2-6)-fructan exohydrolase , levanase , 6-FEH , beta-(2,6)-D-fructan fructohydrolase ) is an enzyme with systematic name (2->6)-beta-D-fructan fructohydrolase .[1] [2] [3] This enzyme catalyses the following chemical reaction
Hydrolysis of terminal, non-reducing (2->6)-linked beta-D-fructofuranose residues in fructans
The best substrates are the levan -type fructans such as 6-kestotriose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside] and 6,6-kestotetraose [beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl-(2->6)-beta-D-fructofuranosyl alpha-D-glucopyranoside].
References [ ]
^ Marx SP, Nösberger J, Frehner M (1997). "Hydrolysis of fructan in grasses: A β-(2-6)-linkage specific fructan-β-fructosidase from stubble of Lolium perenne " . New Phytol . 135 : 279–290. doi :10.1046/j.1469-8137.1997.00642.x .
^ Van den Ende W, De Coninck B, Clerens S, Vergauwen R, Van Laere A (December 2003). "Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel "cell-wall invertase-like" specific 6-FEH from sugar beet (Beta vulgaris L.)" . The Plant Journal . 36 (5): 697–710. doi :10.1046/j.1365-313x.2003.01912.x . PMID 14617070 .
^ Henson CA, Livingston DP (February 1996). "Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructans" . Plant Physiology . 110 (2): 639–44. doi :10.1104/pp.110.2.639 . PMC 157760 . PMID 8742337 .
External links [ ]
Activity Regulation Classification Kinetics Types