(1-4)-alpha-D-glucan exo-4-lyase (1,5-anhydro-D-fructose-forming)

Exo-(1->4)-alpha-D-glucan lyase
EC number4.2.2.13
CAS number148710-18-3
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum

Exo-(1->4)-alpha-D-glucan lyase (EC, alpha-(1->4)-glucan 1,5-anhydro-D-fructose eliminase, alpha-1,4-glucan exo-lyase, alpha-1,4-glucan lyase, GLase) is an enzyme with systematic name (1->4)-alpha-D-glucan exo-4-lyase (1,5-anhydro-D-fructose-forming).[1][2][3][4][5][6][7] This enzyme catalyses the following chemical reaction

linear alpha-glucan (n-1) 1,5-anhydro-D-fructose + D-glucose

The enzyme catalyses the sequential degradation of (1->4)-alpha-D-glucans from the non-reducing end.


  1. ^ Yu S, Kenne L, Pedersén M (March 1993). "Alpha-1,4-glucan lyase, a new class of starch/glycogen degrading enzyme. I. Efficient purification and characterization from red seaweeds". Biochimica et Biophysica Acta (BBA) - General Subjects. 1156 (3): 313–20. doi:10.1016/0304-4165(93)90049-e. PMID 8461323.
  2. ^ Yu S, Pedersén M (1993). "Alpha-1,4-glucan lyase, a new class of starch/glycogen-degrading enzyme. II. Subcellular localization and partial amino-acid sequence". Planta. 191 (1): 137–42. doi:10.1007/bf00240905. PMID 7763826.
  3. ^ Yu S, Ahmad T, Kenne L, Pedersén M (May 1995). "alpha-1,4-Glucan lyase, a new class of starch/glycogen degrading enzyme. III. Substrate specificity, mode of action, and cleavage mechanism". Biochimica et Biophysica Acta (BBA) - General Subjects. 1244 (1): 1–9. doi:10.1016/0304-4165(94)00202-9. PMID 7766642.
  4. ^ Yu S, Christensen TM, Kragh KM, Bojsen K, Marcussen J (May 1997). "Efficient purification, characterization and partial amino acid sequencing of two alpha-1,4-glucan lyases from fungi". Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology. 1339 (2): 311–20. doi:10.1016/s0167-4838(97)00014-9. PMID 9187252.
  5. ^ Yu S, Bojsen K, Svensson B, Marcussen J (August 1999). "alpha-1,4-glucan lyases producing 1,5-anhydro-D-fructose from starch and glycogen have sequence similarity to alpha-glucosidases". Biochimica et Biophysica Acta. 1433 (1–2): 1–15. doi:10.1016/s0167-4838(99)00152-1. PMID 10446355.
  6. ^ Lee SS, Yu S, Withers SG (May 2002). "alpha-1,4-Glucan lyase performs a trans-elimination via a nucleophilic displacement followed by a syn-elimination". Journal of the American Chemical Society. 124 (18): 4948–9. doi:10.1021/ja0255610. PMID 11982345.
  7. ^ Lee SS, Yu S, Withers SG (November 2003). "Detailed dissection of a new mechanism for glycoside cleavage: alpha-1,4-glucan lyase". Biochemistry. 42 (44): 13081–90. doi:10.1021/bi035189g. PMID 14596624.

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