(1-3)-(1-6)-mannosyl-oligosaccharide alpha-D-mannohydrolase

Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase
EC no.
CAS no.82047-77-6
IntEnzIntEnz view
ExPASyNiceZyme view
MetaCycmetabolic pathway
PDB structuresRCSB PDB PDBe PDBsum

Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase (EC, mannosidase II, exo-1,3-1,6-alpha-mannosidase, alpha-D-mannosidase II, alpha-mannosidase II, alpha1-3,6-mannosidase, GlcNAc transferase I-dependent alpha1,3[alpha1,6]mannosidase, Golgi alpha-mannosidase II, ManII, 1,3(1,6)-alpha-D-mannosidase, 1,3-(1,6-)mannosyl-oligosaccharide alpha-D-mannohydrolase) is an enzyme with systematic name (1->3)-(1->6)-mannosyl-oligosaccharide alpha-D-mannohydrolase.[1][2][3][4] This enzyme catalyses the following chemical reaction

Hydrolysis of the terminal (1->3)- and (1->6)-linked alpha-D-mannose residues in the mannosyl-oligosaccharide Man5(GlcNAc)3

This enzyme is involved in the synthesis of glycoproteins. It is a key enzyme of N-linked Glycan processing and is inhibited by small molecule swainsonine.[5]


  1. ^ Harpaz N, Schachter H (May 1980). "Control of glycoprotein synthesis. Processing of asparagine-linked oligosaccharides by one or more rat liver Golgi alpha-D-mannosidases dependent on the prior action of UDP-N-acetylglucosamine: alpha-D-mannoside beta 2-N-acetylglucosaminyltransferase I". The Journal of Biological Chemistry. 255 (10): 4894–902. PMID 6445359.
  2. ^ Tulsiani DR, Hubbard SC, Robbins PW, Touster O (Apr 1982). "alpha-D-Mannosidases of rat liver Golgi membranes. Mannosidase II is the GlcNAcMAN5-cleaving enzyme in glycoprotein biosynthesis and mannosidases Ia and IB are the enzymes converting Man9 precursors to Man5 intermediates". The Journal of Biological Chemistry. 257 (7): 3660–8. PMID 7061502.
  3. ^ Tulsiani DR, Opheim DJ, Touster O (May 1977). "Purification and characterization of alpha-D-mannosidase from rat liver golgi membranes". The Journal of Biological Chemistry. 252 (10): 3227–33. PMID 863880.
  4. ^ Athanasopoulos VI, Niranjan K, Rastall RA (Mar 2005). "The production, purification and characterisation of two novel alpha-D-mannosidases from Aspergillus phoenicis". Carbohydrate Research. 340 (4): 609–17. doi:10.1016/j.carres.2005.01.005. PMID 15721331.
  5. ^ van den Elsen, J.M., Kuntz, D.A., Rose, D.R., "Structure of Golgi alpha-mannosidase II: a target for inhibition of growth and metastasis of cancer cells", European Molecular Biology Organization Journal, 20(12): 3008–3017.

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