Lichenase (EC22.214.171.124, licheninase, beta-(1->4)-D-glucan 4-glucanohydrolase, 1,3, 1,4-beta-glucan endohydrolase, 1,3, 1,4-beta-glucan 4-glucanohydrolase, 1,3-1,4-beta-D-glucan 4-glucanohydrolase) is an enzyme with systematic name(1->3)-(1->4)-beta-D-glucan 4-glucanohydrolase. It was named after its activity in on lichenin (a form of mixed-linkage glucan).
The best-characterised variant of this of enzyme is Bacillus subtilis lichenase, which is used as a molecular biology tool in determining the structure of mixed-linkage glucans. This variant cleaves (1,4) bonds that immediately follow a (1,3) bond.
Other lichenases have different specificities, for example Aspergillus japonicus lichenase cleaves (1,4) bonds that immediately precede a (1,3) bond.
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